What I bring to the table?
In vitro assays
Testing the potential novel therapeutics for safety and efficacy
In vitro ion channels screen:Â hERG assay
In vivo models
Testing the potential therapeutics in animal models
Pharmacological and safety screening of novel potential therapeutics in various
animal models (e.g. Morris water maze, T-maze, etc)
Patch-Clamp/ Slice electrophysiology
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Electrophysiological analysis
Whole-cell current clamp and voltage-clamp recordings to measure several electrophysiological and biophysical properties of the neuronal membrane and circuit interrogations.
Single cell RNA sequencing
Next generation sequencing methods
Hands-on experience with single-cell transcriptomics techniques such as 10X,
PatchSeq and a basic understanding of related data analysis.
Virus injections/ Surgeries/biposies
Surgical procedures and stereotaxic injections
Removal of different brain regions (hippocampus, cerebellum, cortex) for metabolomic/lipidomic analysis, extraction of other organs for IHC and WB. Stereotaxic injection of viruses and surgical implantation of micro prism for two-photon microscopy experiments.
Immunohistochemistry/ Fluorescence microscopy
Imaging techniques
Various microscopy techniques. Familiar with the use of Confocal microscope. Hands-on experience with use of analysis software such as Imaris and Image J
Primary culture
Primary culture: Brain to heart!
Cultured dorsal root ganglion neurons, cortical neurons, CA1 hippocampal neurons, and cardiac myocytes from embryonic and neonatal mice and rats.
Calcium imaging/Two photon
Neuronal activity using Genetically Encoded Calcium Indicators
Micro prism implantation and imaging using 2P microscope.